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Literature summary for 2.4.2.2 extracted from
- Dynamic modelling of phosphorolytic cleavage catalyzed by pyrimidine-nucleoside phosphorylase (2019), Processes, 7, 380 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of N-terminally His6-tagged Py-NPase in Escherichia coli strain BL21 | Parageobacillus thermoglucosidasius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate | Parageobacillus thermoglucosidasius | - |
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose | - |
r |  |
| 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate | Parageobacillus thermoglucosidasius | - |
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose | - |
r | |
| 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate | Parageobacillus thermoglucosidasius | - |
uracil + 2-deoxy-beta-D-arabinofuranose | - |
r | |
| 2'-deoxythymidine + phosphate | Parageobacillus thermoglucosidasius | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-thymidine + phosphate | Parageobacillus thermoglucosidasius | - |
5-bromo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-uridine + phosphate | Parageobacillus thermoglucosidasius | - |
5-bromo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-thymidine + phosphate | Parageobacillus thermoglucosidasius | - |
5-iodo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-uridine + phosphate | Parageobacillus thermoglucosidasius | - |
5-iodo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-methyluridine + phosphate | Parageobacillus thermoglucosidasius | - |
5-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | Parageobacillus thermoglucosidasius | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | Parageobacillus thermoglucosidasius | - |
thymine + alpha-D-ribose 1-phosphate | - |
r | |
| uridine + phosphate | Parageobacillus thermoglucosidasius | - |
uracil + alpha-D-ribose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Parageobacillus thermoglucosidasius | A0A178U4P5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged Py-NPase from Escherichia coli strain BL21 by nickel affinity chromatography | Parageobacillus thermoglucosidasius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.46 | - |
purified recombinant His-tagged enzyme, pH 7.0, 25°C | Parageobacillus thermoglucosidasius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate | - |
Parageobacillus thermoglucosidasius | uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose | - |
r | |
| 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate | - |
Parageobacillus thermoglucosidasius | uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose | - |
r | |
| 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate | - |
Parageobacillus thermoglucosidasius | uracil + 2-deoxy-beta-D-arabinofuranose | - |
r | |
| 2'-deoxythymidine + phosphate | - |
Parageobacillus thermoglucosidasius | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-thymidine + phosphate | - |
Parageobacillus thermoglucosidasius | 5-bromo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-uridine + phosphate | - |
Parageobacillus thermoglucosidasius | 5-bromo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-thymidine + phosphate | - |
Parageobacillus thermoglucosidasius | 5-iodo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-uridine + phosphate | - |
Parageobacillus thermoglucosidasius | 5-iodo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-methyluridine + phosphate | - |
Parageobacillus thermoglucosidasius | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | - |
Parageobacillus thermoglucosidasius | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | - |
Parageobacillus thermoglucosidasius | thymine + alpha-D-ribose 1-phosphate | - |
r | |
| uridine + phosphate | - |
Parageobacillus thermoglucosidasius | uracil + alpha-D-ribose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47600, about, sequence calculation | Parageobacillus thermoglucosidasius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Py-NPase | - |
Parageobacillus thermoglucosidasius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Parageobacillus thermoglucosidasius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Parageobacillus thermoglucosidasius |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | dynamic modelling of phosphorolytic cleavage catalyzed by pyrimidine-nucleoside phosphorylase, differential-dynamical model, kinetics, overview. Py-NPase-catalyzed phosphorylic cleavage reactions are reversible reactions proceeding towards a dynamic equilibrium. The reaction trajectory until equilibrium does not only depend on physical parameters, like temperature and pressure, but also on enzyme concentration, the concentration of substrates, or the presence of products | Parageobacillus thermoglucosidasius |
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Release 2023.1 (January 2023)
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